Measurement of chemically-induced cell proliferation in specific rodent target organs

technical notes for the CIIT Workshop on Cell Replication, September, 1989
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Chemical Industry Institute of Toxicology , Research Triangle Park, NC
Cell proliferation -- Measurement -- Technique -- Congr
Statementedited by Thomas Goldsworthy ... [et al.]
GenreCongresses.
ContributionsGoldsworthy, Thomas Lester., Chemical Industry Institute of Toxicology.
The Physical Object
Pagination99 leaves :
ID Numbers
Open LibraryOL17018311M
OCLC/WorldCa34667461

Details Measurement of chemically-induced cell proliferation in specific rodent target organs EPUB

Guidelines for measuring chemically induced cell proliferation in specific rodent target organs. Goldsworthy TL, Morgan KT, Popp JA, Butterworth BE. A larger data base will be required to relate the extent, duration, and nature of chemically induced cell proliferation to carcinogenic potential and to establish the importance of this end point in chemical by: ELDRIDGE SR, TILBURY LF, GOLDSWORTHY TL, et al.: Mea­surement of chemically induced cell proliferation in rodent liver and kidney: a comparison of 5-bromo-2'deoxyuri­dine and [3H]thymidine administered by injection or os­motic pump.

Chemically induced proliferation has been demonstrated to play a role in the carcinogenic process. A wide range of procedures and techniques are currently being used to define the quantitative relationship between the extent and duration of chemically induced cell proliferation and carcinogenic potential in different species and target by: The target organ specificities of cell proliferation and histopathological lesion induction by 5 carcinogens having different target organs were evaluated using a multiorgan carcinogenesis bioassay.

Measurement ofchemically induced cell proliferation in rodent liver and kidney: a comparison of5-bromo-2'-deoxyuridine and [3H]thymidine administered by injection or osmotic pump. Preparation ofisolated rat liver : J A Styles.

Goldsworthy, K. Morgan, J.

Description Measurement of chemically-induced cell proliferation in specific rodent target organs PDF

Popp, B. Butterworth (4th edn), Measurement of Chemically-Induced Cell Proliferation in Specific Rodent Target Organs, Chemical Industry Institute of Toxicology, BoxResearch Triangle Park, North Carolina (), p.

72 4. M.J Cited by: 8. A segment of duodenum, an organ with a high rate of cell proliferation, was included from each rat to confirm delivery of the DNA precursor. The location of GSTP in the liver was determined by using an antirat GSTP polyclonal antibody (Biotrin, Dublin, Ireland) and Dako Envision alkaline phosphatase goat antirabbit (K; Dako Corp.).Cited by:   Measurement of chemically induced cell proliferation in rodent liver and kidney: a comparison of 5-bromo-2'-deoxyuridine and [3H]thymidine administered by injection or osmotic pump.

The mitogen-activated protein kinase signalling pathway is a central regulator of tumor growth, which is constitutively activated in chemically induced mouse liver tumors. In about 30–50% of Cited by:   Measurement of chemically induced cell proliferation in specific rodent target organs.

Chemically Induced Cell Proliferation, pp 4 — 13, 61 — Google ScholarCited by:   Goldsworthy TL, Morgan KT, Popp JA, Butterworth, BE () Guidelines for measuring chemically induced cell proliferation in specific rodent target organs.

In: Butterworth BE, Slaga TJ, Farland W, McCain W (eds) Chemically induced cell proliferation: implications for risk assessment. Wiley-Liss, New York, pp –Cited by: Byron E. Butterworth's research works with 5, citations and 3, reads, including: Expression of the hepatocyte growth factor and c‐MET genes during furan‐induced regenerative cell.

Chapter 15—Assays for Cell Viability, Proliferation and Function Overview of Probes for Cell Viability, Cell Proliferation and Live-Cell Function—Section ; Viability and Cytotoxicity Assay Reagents—Section ; Viability and Cytotoxicity Assay Kits for Diverse Cell Types—Section Measurement of chemically induced cell proliferation in rodent liver and kidney: a comparison of 5-bromo-2′deoxyuridine and 3 H-thymidine administered by injection or osmotic pump Eldridge, SR; Tilbury, LF; Goldsworthy, TL.

In the current monograph, studies on the effect of acrylamide on DNA reactivity and on altered cell growth in the target tissues in the rat are reported.

DNA synthesis was examined in F rats treated with acrylamide (0, 2, or 15 mg/kg/day) for 7, 14, or 28 by:   The standard method for assessment of cell proliferation in paraffin-embedded tissue sections is 5-bromodeoxyuridine (BrdU) immunohistochemistry (IHC).

BrdU can be administered to laboratory animals via IP injections, is readily incorporated into nuclei during the DNA synthetic phase of the cell cycle, and is detected with an anti-BrdU by:   Evaluation of Cytotoxicity, Cell Proliferation, and Genotoxicity Induced by p-Cresidine in Hetero- and Nullizygous Transgenic p53 Mice Don A.

Delker The Dow Chemical Company, Health and Environmental Research Laboratory, Building, Midland, Michigan Cited by: 9. C12 and C17 are two types of bladder-specific stromal cells with high expression of Bmp4 and Wnt2.

C12 expresses high levels of Cxcl12 and the proliferation marker Ifitm1, while C17 expresses a high level of Bmp5. Both the testis and neonatal heart contribute to C9 stromal by: Measurement of ploidy and cell proliferation in the rodent liver. J A Styles; Published: 1 December ; Site-specific cell proliferation in renal tubular cells by the renal tubular carcinogen tris(2,3-dibromopropyl)phosphate.

Role of chemically induced cell proliferation in carcinogenesis and its use in health risk assessment. R G Croy. Effect ofglycerol on cell kinetics and tumorigenesis in mouse lung following urethane administration. Evaluation of dichloromethane as an inducer of DNA synthesis in the B6C3Fj mouse liver.

Guidelines for measuring chemically induced cell proliferation in specific rodent target organs. In: Chemically Induced Cell. Any information on the dose-response nature of these effects, especially cell proliferation, should be included in assessments of risk where possible, although, as Konishi et al.

() and Ward et al. () emphasize, the target cells for proliferative activity associated with carcinogenesis might not be the total parenchymal tissue. Furthermore, metabolic modulations that reduce the formation of DNA adducts in target tissues of rodents will also reduce tumor risk.

DNA adducts may form in organs that do not develop tumors, indicating that species- and tissue-specific risk factors (for example cell proliferation. Chen D, Livne-bar I, Vanderluit JL, Slack RS, Agochiya M, Bremner R () Cell-specific effects of RB or RB/p loss on retinal development implicate an intrinsically death-resistant cell Cited by:   Molecular mechanisms and cell-cell interactions in peroxisome proliferator-induced effects in rodent liver.

Download Measurement of chemically-induced cell proliferation in specific rodent target organs FB2

Kupffer cells have been shown to be rapidly activated by peroxisome proliferators, generate oxidants, and release mitogenic cytokines that stimulate proliferation of parenchymal by: alamarBlue™ Cell Viability Reagent, PrestoBlue™ Cell Viability Reagent, and CyQUANT™ Cell Proliferation Assay Kit (Cat.

C) have been validated for use with the AlgiMatrix™ 3D Culture System and should also work with other 3D culture systems. Whether acute or long term, exercise elicits remodeling of the heart that includes an increase in heart size and alterations in cellular and molecular signaling pathways.

Vega et al. summarize the morphological characteristics and cellular signaling pathways associated with the heart’s ability to adapt when subjected to exercise by: Furthermore, chemically induced cell proliferation of target cells might play an important role in the induction of some tumors, and cell proliferation should be measured when that is appropriate.

It is difficult to conceive how similar information could be gathered in humans. Measurement of chemically induced cell proliferation in rodent liver and kidney: a comparison of 5-bromo-2'deoxyuridine and [[sup.3]H]thymidine administered by injection or osmotic pump.

Carcinogenesis (). LUNG CANCER CHEMOPREVENTION IN RODENT MODELS OF CHEMICAL CARCINOGENESIS Chemically-induced lung tumors have been widely used to identify drugs and botanically-derived agents that may be effective for chemoprevention. Chemoprevention can be defined as the use of chemo- or dietary agents to prevent tumor formation or progression.

Specific Organ/System Toxicity: the Liver soft tissues. Already, such instruments are able to measure 4-mm 'slices' through the liver and measurement of l-mm 'slices' should be possible in the near future. Morphology A number of useful morphological indices of liver damage are given in File Size: 7MB.

INTRODUCTION. Muscle tissue repair is a complex biological process that crucially involves activation of stem cells. Skeletal muscle contains two different stem cell types: 1) myogenic stem cells, so-called satellite cells (SCs), that reside beneath the basal lamina of muscle fibers (Mauro, ) and express both NCAM/CD56 and early myogenic cell markers such as M-cadherin, Pax7, and Myf5.Privacy Policy | Environmental Health Perspectives, TW Alexander Drive, Durham, NC | Environmental Health Perspectives, TW Alexander Drive, Durham, NC COVID pandemic in Atlanta - The ongoing pandemic for the city of Atlanta, Georgia.

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